Journal article
Analysis of the breast cancer methylome using formalin-fixed paraffin-embedded tumour
EM Wong, JHE Joo, CA McLean, L Baglietto, DR English, G Severi, HC Wu, MB Terry, JL Hopper, RL Milne, GG Giles, MC Southey
Breast Cancer Research and Treatment | SPRINGER | Published : 2016
Abstract
Purpose: Aberrant DNA methylation occurs frequently in breast carcinogenesis. Tools for translational epigenetic studies of breast cancer involving formalin-fixed paraffin-embedded (FFPE) human tissues have now been developed. Few studies have measured genome-wide methylation in DNA derived from paraffin-embedded tumour tissues and compared the DNA methylation in corresponding adjacent non-tumour ductal epithelium (ADJNT). These studies are technically challenging due to the spectrum of breast cancer pathologies, the variable suitability of DNA extracted from FFPE material and the difficulties in identifying ADJNT. We assessed the suitability of FFPE breast cancer material for genome-wide DN..
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Awarded by Cancer Council Victoria
Funding Acknowledgements
This work was further supported by the National Health and Medical Research Council (NHMRC; APP1011618 and APP1026892) and The Victorian Breast Cancer Research Consortium. MCS is a NHMRC Senior Research Fellow and JLH is a NHMRC Senior Principal Research Fellow. EMW is a recipient of the Dyason Fellowship.